Bacterial iron-sulfur proteins.
نویسندگان
چکیده
منابع مشابه
Iron-sulfur proteins: spin-coupling model for three-iron clusters.
Recent Mössbauer and EPR studies of two ferredoxins and of aconitase have given evidence for a three-iron cluster, probably of a [3Fe-3S] type. The studies of the oxidized EPR-active centers have shown that the three iron sites are characterized by significantly different magnetic hyperfine coupling constants. For the ferredoxin from Azotobacter vinelandii, for instance, we have observed A1 = -...
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The compound (Et(4)N)(2)[Fe(4)S(4)(SCH(2)Ph)(4)] has been prepared and its structure determined by x-ray diffraction. The Fe(4)S(4) core of the anion possesses a configuration of D(2d) symmetry that is closely related to the Fe(4)S(4) active-site structures of the high-potential iron protein from Chromatium and the ferredoxin from Micrococcus aerogenes. Electronic properties of the tetrameric a...
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In an investigation concerned with the solubilization and characterization of membrane-bound iron-sulfur proteins of photosynthetic bacteria, succinate dehydrogenase was solubilized from Rhodospirillum rubrum chromatophores by the detergent lauryl dimethylamine oxide (LDAO) or by an alkaline washing treatment. The LDAO-solubilized enzyme was purified by a combination of DEAE-cellulose and Sepha...
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The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains little iron and no acid-labile sulfide. An [Fe-S] cluster was reconstituted on Ap...
متن کاملCluster characterization in iron-sulfur proteins by magnetic circular dichroism.
We report magnetic circular dichroism (MCD) spectra of 4-Fe iron-sulfur clusters in the iron-sulfur proteins Chromatium high-potential iron protein (HIPIP), Bacillus stearothermophilus ferredoxin and Clostridium pasteurianum ferredoxin. The MCD is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. The spectra obtained are compar...
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ژورنال
عنوان ژورنال: Microbiological Reviews
سال: 1979
ISSN: 0146-0749
DOI: 10.1128/mmbr.43.3.384-421.1979